Figure 5.

αR350W, αH354R, and αG355R reduce the Na⁺ self-inhibition response. A, C, E, and G, representative recordings in oocytes expressing WT or mutant ENaCs to show the Na⁺ self-inhibition response. Oocytes were clamped at −100 mV, whereas bath Na⁺ concentration was increased from 1 mm (NaCl-1, gray bar) to 110 mm (NaCl-110, black bar). Traces were superimposed with the same time and current scales. B, D, F, and H, I_ss/I_peak represents the magnitude of Na⁺ self-inhibition. Values were obtained from amiloride-sensitive I_ss and I_peak. Horizontal bars are mean ± S.D. Data were collected from three batches of oocytes. The p values were from Student's t tests, NS, not significant.