Figure 8.
Transcriptomic analysis of EndoC-βH1 cells in response to cytokine exposure. A, overlap of distributions of mRNA levels in the EndoC-βH1 transcriptome with (red) or without (black) 1× cytokine exposure for 24 h. Black line, SLC30A8 mRNA level in EndoC-βH cells. Dashed magenta line, a 10-fold increase of the SLC30A8 mRNA level in human pancreatic β-cells (83). B, mRNA levels of ZnT (SLC30) and ZIP (SLC39) gene families in EndoC-βH1 cells. Error bars, S.D. of three biological replicates. C, correlation of mRNA levels with and without cytokine exposure along a diagonal line. Off-diagonal data points, cytokine-induced transcriptional changes. Red data points, LMP2, LMP7, and MECl-1 transcripts. Blue data points, other UPR-related transcripts listed in Table 1.