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. 2019 Sep 19;294(45):16650–16662. doi: 10.1074/jbc.RA118.006961

Figure 4.

Figure 4.

PLCγ1 is a cytosolic and PLCδ4 is a nuclear protein. A, representative confocal images of SKHep-1 cells before and after EGF stimulation showing PLCγ1 in red, EGFR in green, and TO-PRO nuclear dye in blue. Merged images reveal that PLCγ1 is predominantly expressed outside of the nucleus in both conditions (n = 15 cells). B, representative confocal images of control and EGF-stimulated SKHep-1 cells showing PLCδ4 in red, which is exclusively intra-nuclear (n = 15 cells). C, Western blotting showing PLCγ1 and PLCδ4 expressions in non-nuclear and nuclear fractions from control or EGF-stimulated hepatocytes. α-Tubulin and lamin B1 were used as purity controls for nuclear and non-nuclear fractions. Indicated kDa values represent the position of the reference bands from pre-stained protein standard. Subcellular localizations of PLCγ1 and PLCδ4 in hepatocytes are not altered by EGF stimulation for 10 min (n = 3). D, tracings of normalized amounts of PLCγ1 in non-nuclear and nuclear fractions of SKHep-1 cells upon EGF stimulation with different time points, quantified from Western blotting experiments (n = 3).