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Relative activities of the WT and mutant enzymes of Endo-CoM toward PA substrates
Relative activity (%) compared with the activity of the WT enzyme for PA-fucosyl sialobiantennary (8.03 μmol/min/mg) is shown.
| Enzyme | PA-fucosyl sialobiantennary | PA-sialobiantennary |
|---|---|---|
| WT | 100 | 0.99 |
| E156Q | 1.27 | –a |
| D154N/E156Q | 1.20 | 0.02 |
| N193A | 184 | 0.52 |
| Y216A | 3.45 | –a |
| R218A | 14.9 | 2.24 |
| W253A | 63.0 | 0.18 |
a – indicates not determined.
