Figure 3.

Assay optimization for the screening of peptides disrupting 5-HT_2AR–CB₁R heteromers. A, schematic representation of the CMV-driven NanoBiT N-terminally tagged CB₁ and 5-HT_2A receptors. Shown is a comparison between N-terminal or C-terminal tagging to assess CB₁R and 5-HT_2A receptor homomers (B) or 5-HT_2AR–CB₁R heteromers (C). Data are mean ± S.E. (error bars) (n = 3) of -fold change, calculated as the ratio between each condition and the individual receptor construct with the highest luminescence background. Statistical significance was evaluated by unpaired t tests between groups followed by Holk–Sidak corrections for multiple comparison (*, p ≤ 0.05; ****, p ≤ 0.0001). CMV, human cytomegalovirus immediate-early promoter.