Figure 1.

The expression of FTO was reduced, and the m⁶A levels were increased in cisplatin-induced kidney injury. Kidney samples were collected 3 days after cisplatin administration. A, the expression of FTO in kidneys was assessed by Western blotting. B, dot blot was performed to assess the level of total RNA m⁶A in kidneys. C, Western blotting was performed to measure FTO expression in human proximal tubular epithelial cells (HK2 cells). Protein samples were extracted from HK2 cells 24 h after cisplatin treatment. D, the m⁶A level in HK2 cells was assessed by dot blot. The data represent means ± S.D., and the results are representative of three independent experiments. Veh, vehicle; Cis, cisplatin; Con, control; MB, methylene blue.