Figure 6.

Knockdown of FTO promoted apoptosis in cisplatin-treated HK2 cells and enhanced m6A levels. HK2 cells were transfected with nonsense siRNA (Si-NC) or the Si-FTO. 24 h after the transfection, HK2 cells were stimulated with 10 μmol/liter cisplatin for another 24 h. A, the expression of FTO, Bcl-2, Bax, and Cleaved Caspase-3 in HK2 cells was assessed by Western blotting and then were quantified. B, TUNEL staining was performed to measure cell apoptosis (original magnification, ×400; bar, 200 μm) and then was quantified. C, dot blot was used to assess the m6A level in HK2 cells, which was further quantified. Methylene blue (MB) staining was used to measure the quantity of RNA in membrane blots. The data represent means ± S.D., and results are representative of three independent experiments. Cis, cisplatin; NS, normal saline.