Figure 8.

Overexpression of METTL14 promoted apoptosis and enhanced m6A levels in vitro. A, kidney samples were collected 3 days after cisplatin administration. The expression of METTL14 in kidneys was assessed by Western blotting. B, HK2 cells were transfected with empty vectors (pCN) or METTL14 (pMETTL14) plasmids, 24 h after the transfection, HK2 cells were stimulated with 10 μmol/liter cisplatin for another 24 h. The expression of METTL14, Bcl-2, Bax, and Cleaved Caspase-3 in HK2 cells was assessed by Western blotting and then were quantified. C, TUNEL staining was performed to measure cell apoptosis (original magnification, ×400; bar, 200 μm) and then was quantified. D, dot blot was used to assess the m⁶A level in HK2 cells, which was further quantified. Methylene blue (MB) staining was used to measure the quantity of RNA in membrane blots. The data represent means ± S.D., and the results are representative of three independent experiments. Cis, cisplatin; NS, normal saline; Veh, vehicle.