Figure 2.

TIP60 inhibition reduces ΔNp63α expression. TIP60 catalytic activity is important for regulation of ΔNp63α transcript levels and protein stability. A, A431 cells treated with vehicle (Veh, DMSO) or with the TIP60-specific inhibitor NU9056 (100 μm) for 24 h. The changes in protein expression and transcript levels of ΔNp63α and TIP60 were measured by immunoblot analyses (left panel) and TaqMan-based real-time PCR (right panel), respectively (*p ≤ 0.05). Error bars indicate 1 S.D. B and C, H1299-ΔNp63α stable cells were transfected with TIP60 WT (B) or TIP60 DN mutant (C), followed by treatment with 100 μg/ml CHX for 1, 2, or 4 h, along with an NT control. Expression of ΔNp63α and TIP60 was confirmed via immunoblot analysis (left panels). The -fold change in ΔNp63α protein levels, normalized to β-actin and relative to the NT condition, is plotted in the right panels.