Figure 3.

TIP60 reduces ΔNp63α ubiquitination and proteasomal degradation. H1299 cells were transfected with ΔNp63α and HA-ubiquitin (HA-Ub) and either EV or TIP60. 24 h post-transfection, cells were treated with 20 μm lactacystin for 4 h. A and B, whole-cell extracts were subjected to immunoprecipitation (IP) using anti-p63 (A) or anti-HA (B) antibodies. The bottom three blots show immunoblots for ΔNp63α, TIP60, and β-actin of the whole-cell extract (WCE) used for IP. WB, Western blot. C, lenti-ΔNp63α H1299 cells were transfected with either EV or TIP60 and treated with the indicated concentrations of lactacystin for 4 h. ΔNp63α and TIP60 were detected by immunoblot with anti-p63 and anti-TIP60 antibodies. The -fold change in protein level, normalized to β-actin relative to EV, is listed above each band.