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In vitro activity assay of the naked or O-GlcNAc sOGT (Figs. S2–S5)
| Measurements | Naked sOGT | O-GlcNAc sOGT |
|---|---|---|
| Using CK3k peptide | ||
| Yields | 63.6% | 65.4% |
| Using FITC-YAVVPVSK peptide | ||
| Yields | 22.9% | 22.4% |
| Km (μm) | 175.7 ± 18.26 | 180.5 ± 26.17 |
| Vmax (μm/min) | 48.54 ± 18.26 | 27.27 ± 26.17 |
