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. 2019 Sep 13;294(45):17017–17030. doi: 10.1074/jbc.RA119.008947

Figure 6.

Figure 6.

Rotation of WT and Arg-finger mutant EhV1 in the presence of a high concentration of Pi. A–D, examples of time course of the rotation. The rotations of WT EhV1 at 1 mm ATP (A), 10 μm ATP (B), 1 mm ATPγS (C), and Arg-finger mutant EhV1 at 1 mm ATP (D) were observed at 10,000 fps in the presence of 20, 200, and 500 mm Pi. E–H, Pi concentration dependences of rotation velocity. The red open circles and small filled circles indicate average velocity and velocities of individual molecules in the presence of Pi, respectively. I–L, ionic strength dependences of rotation velocity. The red open circles and small filled circles indicate average velocity and velocities of individual molecules in the presence of Pi, respectively. The green open squares and small filled squares in I and J indicate average velocity and velocities of individual molecules in the presence of KCl, which is added instead of Pi as an ion source. Error bars, S.D.