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. 2019 Aug 2;294(45):16511–16524. doi: 10.1074/jbc.RA119.009654

Figure 4.

Figure 4.

Multiubiquitylation of RPN13 in response to 10-min S1 w/o. A, AgDD-Ubn conjugates captured by UBA pulldown from mock– and S1 w/o–treated AgDD cells. B, pretreatment with the deubiquitinase domain USP2-cc pretreatment eliminates UBA capture of HMW AgDD bands. C, RPN13-Ubn conjugates captured by UBA pulldown from mock– and S1 w/o–treated AgDD cells. D, USP2-cc pretreatment eliminates UBA capture of HMW RPN13 bands. Immunoblots were probed with the indicated antibodies.