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. 2019 Nov 12;9:16573. doi: 10.1038/s41598-019-52642-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Antagonizing the glycine and GABA receptors increased the excitability of the GRPR neurons. (a) Current induced action potentials from GRPR neurons. The upper trace in a1 is the induced action potential and the lower trace is the stimulating current pulse (duration 500 ms). a2 shows the induced action potential after strychnine and bicuculline treatment. (b) Comparison of the rheobase between control group (n = 15) and the antagonist treated neurons (n = 5) (two-tailed T-test, p = 0.0337). (c) Input resistance comparison between control group (n = 16) and the antagonist treated neurons (n = 5) (two-tailed T-test, p = 0.0005). (d) Resting membrane potentials in the control group (n = 13) and the antagonist treated neurons (n = 5).