Table 1.

Histological evaluation of all samples for which material was available

Patient	Specimen	Grade	Cellularity	Vessels	Mitotic activity	Necrosis	MYCN-FISH	MYCN-IHC	GFAP	EMA	H3K27me3	Ki67 up to (%)
1	Primary	II	Low	No MP	Low	No	Amp	++	+++	Dot-like	Retained	3
1	Relapse 1	III	Low and high	No MP	Brisk	No	Amp	++  to +++	+ with PE	Dot-like	Retained	20
1	Relapse 2	III	High	MP	Brisk	Present	Amp	+++	+ with PE	Dot-like	Retained	30
1	Relapse 3	III	High	MP	Brisk	Present	Amp	+++	+ with PE	Dot-like	Retained	20
1	Relapse 4	III	High	MP	Brisk	Present	Amp	+++	+ with PE	Dot-like	Retained	30
2	Primary	III	High	No MP	Moderate	No	Amp	+++	+++	Dot-like	Retained	15
2	Relapse 1	III	High	MP	Moderate	No	Amp	+++	+++	Dot-like	Retained	15
2	Relapse 2	III	Low and high	MP	Brisk	Present	Amp	++ to +++	++ with PE	Dot-like	Retained	25
2	Relapse 3	III	High	MP	Brisk	Present	Amp	+++	+ with PE	Dot-like	Retained	25
5	Primary	III	Low and high	MP	Brisk	No	Amp	++ to +++	++ with PE	Dot-like	Retained	30
10	Primary	III	Low and high	MP	Brisk	Present	Amp	++ to +++	++	Dot-like	Retained	60
12	Primary	III	Low and high	MP	Brisk	No	ND	++ to +++	++ with PE	Dot-like	Retained	40

MP microvascular proliferation, PE perivascular enhancement

+ focal, ++ weak to moderate and widespread, +++ strong and widespread