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. 2019 Nov 12;9:16647. doi: 10.1038/s41598-019-53145-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Photographs of pancreas, liver, and tumor sections stained with hematoxylin-eosin (HE) to evaluate the impact of the insulin and chemotherapeutics on the tissues. The figure presents an organ from a control mouse and mouse treated with insulin and FU together (group 5). There are no significant changes in the morphology in the pancreas and liver. The figure of the tumor presents control, insulin, fluorouracil, and insulin together with fluorouracil (group 5) conditions. Sections of fluorouracil and fluorouracil with insulin reveals parts of the necrotic area in comparison to the control. Optical magnification: 200x (pancreas, liver) and 100x (tumor). (B) Illustrative images of CTCs detected in mouse blood. CTCs with a green EpCAM surface staining, a well-preserved morphology, but no red nuclear staining by PI is shown. The figure presents an image from a control mouse and mouse treated with insulin and FU together.