Skip to main content
. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: Proteins. 2019 Jul 24;87(12):1021–1036. doi: 10.1002/prot.25775

Figure 5. Target EU difficulty correlates with server performance in CASP12-like plot.

Figure 5.

A) Scatter of EU difficulty measured by the average of HHscore and LGA_S similarity to templates and server performance measured by average GDT of the top 20 server models. EUs are colored according to assigned categories: TBM-easy (green), TBM-hard (yellow), TBM/FM (gray), and FM (red). Difficult borderline EUs requiring manual assignment are labeled. B) FM target T0990-D1 (left) with relatively high LGA_S and performance scores does not cluster with other FM EUs. A unique loop includes conserved residues (magenta sphere) that typically bind metal. The metal binding residues are absent from the top unrelated structural template (2rt6, right), which adopts a three-helix bundle. C) TBM-hard target T0960-D3 (left) identified a template homolog (5nxf, right) with a similar overall fold (LGA_S 83.2) with relatively low sequence scores (82%, 0.54 coverage). D) TBM/FM target T0958 identified a template homolog (2kim, right) with relatively low sequence scores (81.4%, 0.59 coverage). The template exhibits SSE shifts (LGA_S 69.2) compared to the target. E) TBM/FM target T1008 represents a designed protein structure that by definition is analogous to its top template (5hnwK, right, LGA_S 73.9).