Table 1.
Overview of the primary antibodies used in the present study
| Antibody | Phospho-epitope | Species | Dilution | Source | Product number | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Tissue | In vitro | |||||||||
| IF | IHC | Confocal | STED | TEM | WB | |||||
| Acetylated α-tubulin | Mouse monoclonal | 1:100 | Sigma-Aldrich | T7451 | ||||||
| AT100 | Thr212/Ser214 | Mouse monoclonal | 1:1000 | 1:200 | ThermoScientific | MN1060 | ||||
| AT8 | Ser202/Thr205 | Mouse monoclonal | 1:800 | 1:400 | 1:400 | 1:500/1:1000 | ThermoScientific | MN1020 | ||
| β-III-tubulin | Mouse monoclonal | 1:500 | Merck Millipore | MAB1637 | ||||||
| Calnexin | Rabbit polyclonal | 1:250 | Enzo Life Sciences | ADI-SPA-860 | ||||||
| CHMP2B [4] | Rabbit polyclonal | 1:200 | 1:1000 | Abcam | ab33174 | |||||
| CK1δ | Mouse monoclonal | 1:1000 | 1:100 | 1:100 | 1:1000 | Santa Cruz Biotechnology | sc-55553 | |||
| CK1δ | Rabbit polyclonal | 1:400 | 1:500 | ThermoScientific | PA5-32129 | |||||
| CK1ɛ | Rabbit polyclonal | 1:100 | Santa Cruz Biotechnology | sc-25423 | ||||||
| c-Myc | Mouse monoclonal | 1:50 | Santa Cruz Biotechnology | sc-40 | ||||||
| c-Myc | Chicken polyclonal | 1:50 | Aves Labs | ET-MY100 | ||||||
| CTSD | Rabbit polyclonal | 1:100 | Merck Millipore | 06-467 | ||||||
| EEA1 | Rabbit polyclonal | 1:100 | Cell Signaling | 2411 | ||||||
| GAPDH | Mouse monoclonal | 1:2500 | Merck Millipore | MAB374 | ||||||
| GFAP | Mouse monoclonal | 1:1000 | Sigma-Aldrich | G3893 | ||||||
| GRASP55 | Rabbit polyclonal | 1:100 | Atlas Antibodies | HPA035275 | ||||||
| G3BP | Mouse monoclonal | 1:100 | BD Transduction Laboratories | 611126 | ||||||
| HT7 | Mouse monoclonal | 1:1000 | ThermoScientific | MN1000 | ||||||
| LAMP1 | Rat monoclonal | 1:100 | Abcam | ab25245 | ||||||
| LC3 [34, 46] | Mouse monoclonal | 1:50 | MBL | M152-3 | ||||||
| LIMP2/lpg85 | Rabbit polyclonal | 1:500 | 1:100 | Novus Biologicals | NB400-129 | |||||
| MAP2 | Chicken polyclonal | 1:500 | Abcam | ab5392 | ||||||
| MC1 | Mouse monoclonal | 1:500 | Kind gift from Dr. Peter Davies | |||||||
| peIF2α | Ser51 | Rabbit polyclonal | 1:100 | Sigma-Aldrich | E2152 | |||||
| peIF2α | Ser51 | Rabbit polyclonal | 1:50 | Cell Signaling | 9721 | |||||
| pIRE1α | Ser724 | Rabbit polyclonal | 1:1250 | 1:50 | Novus Biologicals | NB100-2323 | ||||
| pPERK | Thr981 | Rabbit polyclonal | 1:6000 | 1:800 | 1:1000 | Santa Cruz Biotechnology | sc-32577 | |||
| Tau-5 | Mouse monoclonal | 1:1000 | Abcam | ab80579 | ||||||
| VGAT | Rabbit polyclonal | 1:500 | Synaptic Systems | 131002 | ||||||
| VGLUT1 | Guinea pig polyclonal | 1:1000 | Merck Millipore | AB5905 | ||||||
Target protein and species the primary antibody was raised are listed. For phospho-specific antibodies the phospho-epitope is indicated. The dilution used in different experimental approaches, the source and product number are listed. For some antibodies, references to studies validating the antibody are included
IF immunofluorescence, IHC immunohistochemistry, STED stimulated emission depletion (microscopy), TEM transmission electron microscopy, WB Western blotting