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. 2019 Nov 13;39(11):BSR20191692. doi: 10.1042/BSR20191692

Figure 5. Role of the β-catenin PDZ binding motif to transcriptional activity.

Figure 5

293T cells were cotransfected with equal amounts of the indicated mCherry-tagged β-catenin wild-type, mutant (ΔFDTDL) or vector control plasmids along with HA-tagged tcf-4, and either TOPFLASH or FOPFLASH reporters. Total cell lysates were equalized for protein concentration and assayed for luciferase activity 48 h post-transfection (A). Expression of β-catenin and tcf-4 was detected using Western blotting using mCherry of HA monoclonal antibodies, respectively (B) (*P<0.05).