Figure 6.

A. Assessment of the activity of SosAd10 variants with point mutations (alanine substitutions) at conserved residues (37/38, 40/41 and 44/45) within the C‐terminal part. Activity of the constructs was assessed in S. aureus JE2 and compared to the vector control (‐), SosA and SosAd10. Cells were grown exponentially to an OD₆₀₀ of 0.5, serially 10‐fold diluted and plated on TSA (control) or TSA plus inducer (AHT) at increasing concentrations followed by incubation overnight at 37°C. B. Comparison of the activity of the point mutation protein SosA (44A) with WT SosA in S. aureus JE2. Cells were grown as above and plated on TSA or TSA plus 200 ng ml⁻¹ AHT.