Figure 7.

A) HRP consumes H₂O₂ and AR to produce a fluorescent product, resorufin. B) The Michaelis–Menten curves for succinylated HRP (30 pm final concentration) in a bulk solution (black) or within coacervates (red) containing 250 μm AR and varied concentrations of H₂O₂. C) The Michaelis–Menten curves for succinylated HRP (30 pm final concentration) in a bulk solution (black) or within coacervates (red) containing 250 μm H₂O₂ and varied concentrations of AR. The kinetic constants derived from the Michaelis–Menten fits are given in Table 1. The error bars are standard deviations of the rates estimated from duplicates of at least two separate experiments.