Figure 2.

Recommended experiments to test source‐trapping of secreted ligands. (a) Trapping the POI in the source should eliminate the POI in the target tissue. If the response in the target tissue (signaling, target gene expression, etc.) is also dependent on dispersal, the response should be also eliminated. (b) POI trapped in the receiving cells should be eliminated by efficient trapping the POI in the source. (c) The source trapping phenotype in hemizygotes should not be stronger than that in homozygotes. (d) Phenotypes caused by trapping the POI should resemble those observed by the membrane‐tethered version of POI