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. 2019 Aug 23;112(4):1284–1307. doi: 10.1111/mmi.14362

Figure 7.

Figure 7

Isotopic species analysis reveals production of propionyl‐CoA by different pathways. Percentage of all the isotopic species in Krebs cycle, methylcitrate cycle, glyoxylate shunt and valine metabolism intermediates detected in Mtb grown in lactate (A) and pyruvate (B) at the concentration of 0.4%. The charts show the average and standard deviation of four biological replicates from one experiment, representative of two independent experiments. The purple rings at the top of ech metabolite name represent the number of carbon atoms. *p value <0.00001 between propionate M + 3 and the indicated isotopic species of methyl(iso)citrate and methylmalonate semialdehyde. cis‐ACO = cis‐aconitate; ASP = aspartate; FUM = fumarate; GLO = glyoxylate; α‐KG = α‐ketoglutarate; ISOBUT‐CoA = isobutyryl‐CoA; ISOCIT = isocitrate; MAL = malate; 2MC = 2‐methyl‐citrate; 2MIC = 2methyl‐isocitrate; MA‐CoA = methylacryl‐CoA; MMSA = methylmalonyl semialdehyde; α‐KIV = α‐ketoisovalerate; OAA = oxaloacetate; PEP = phosphoenolpyruvate; PRO‐CoA = propionyl‐CoA; SER = serine; SUC = succinate; VAL = valine.