Figure 9.

The assimilation of lactate and pyruvate is similar between H37Rv, CDC1551, Erdman and HN878 strains. A. Growth in lactate, in pyruvate or in glucose as carbon sources. The plots show the average and error (average deviation) of two independent experiments. B and C. Enzymatic activity of isocitrate lyase (B) and isocitrate dehydrogenase (C) measured in cell extracts of Erdman (top panels) and HN878 (bottom panels) grown in lactate, in pyruvate or in glucose as one carbon source. The plots show the average and error (average deviation) of two independent experiments and two technical replicates. The values at the top of the bars represent the fold changes of the activity detected in lactate or pyruvate vs the activity detected in glucose (minimum activity in pyruvate/lactate over maximum activity in glucose). *p value <0.00001; **p value <0.0003; ***p value <0.0002; ****p value <0.003; *****p value <0.008; ******p value <0.05. D. Growth of Mtb strains on solid media supplemented with the specific carbon source and different concentration of 3NP (µM). 5 µl of serial dilutions of 0.1% and 0.4% carbon source pre‐adapted cultures were spotted on the media. The pictures were taken after 30 days of growth, and they are representative of two independent experiments.