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. 2019 Jun 12;59(8):2662–2671. doi: 10.1111/trf.15398

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© 2019 The Authors. Transfusion published by Wiley Periodicals, Inc. on behalf of AABB.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Platelet activation and aggregation of cryopreserved PLTs. (A) The percentage of PLTs expressing activated integrin α_IIbβ₃ in response to stimulation with 32.5 μmol/L TRAP6 was determined by PAC‐1 binding in flow cytometry. (B) Maximal amplitude (%) of PLT aggregation in response to a mix of epinephrine, MeSADP, and TRAP6 is shown. AS‐PCT PLTs () were compared to standard () PLTs before storage (21°C) or after cryopreservation (−80°C; n = 8).

Inline graphic — Platelet activation and aggregation of cryopreserved PLTs. (A) The percentage of PLTs expressing activated integrin α_IIbβ₃ in response to stimulation with 32.5 μmol/L TRAP6 was determined by PAC‐1 binding in flow cytometry. (B) Maximal amplitude (%) of PLT aggregation in response to a mix of epinephrine, MeSADP, and TRAP6 is shown. AS‐PCT PLTs () were compared to standard () PLTs before storage (21°C) or after cryopreservation (−80°C; n = 8).