Figure 1.

CD4⁺ and DN TCRβ⁺ splenocyte frequencies in DKO mice. Spleen cells were prepared and stained for different surface markers. A, Representative flow cytometry plots showing gatings for CD4⁺ and DN splenic TCRβ⁺ cells from CD1d^−/−MHCII^−/− and Jα18^−/−MHCII^−/− mice. B, C, Scatter plots illustrate frequencies among B220⁻CD8⁻ cells (left) and absolute numbers per spleen (right) of CD4⁺ and DN TCRβ⁺ cells. Results are expressed as mean ± SD; each dot represents values from one mouse. ** indicates P < 0.001 and *** P < 0.0001 as calculated by Mann‐Whitney U test (two‐tailed)