Figure 3.

Increased expression of NKG2 receptors on CD4⁺ and DN splenic TCRβ⁺ cells in Jα18^−/−MHCII^−/− compared to CD1d^−/−MHCII^−/− mice. Spleen cells were prepared and stained for different surface markers. A, Flow cytometric plots display the expression patterns of NKG2D and NKG2A/C/E on CD4⁺ and DN splenic TCRβ⁺ cells from CD1d^−/−MHCII^−/− and Jα18^−/−MHCII^−/− mice. B, Frequencies among B220⁻CD8⁻TCRβ⁺ splenocytes gated as in (A) (left) and absolute numbers (right) of NKG2D and NKG2A/C/E expressing CD4⁺ and DN TCRβ⁺ cells. White bars represent CD1d^−/−MHCII^−/−, and black bars represent Jα18^−/−MHCII^−/− DKO mice. Results are expressed as mean ± SD of 8 mice from four independent experiments; * indicates P < 0.001, ** P < 0.001 and **** P < 0.00001 as calculated by Mann‐Whitney U test (two‐tailed). The flow cytometry plots of co‐expression of NKG2D and NKG2A/C/E on CD4⁺ and DN T cells in DKO strains shown in (A) is representative of two experiments