Figure 4.

Expression of surface markers on NKG2D⁺ CD4⁺ and DN TCRβ⁺ splenocytes from DKO mice. Spleen cells were prepared and stained for different surface markers. Representative flow cytometric histograms display the expression of indicated markers on NKG2D⁺ CD4⁺ and NKG2D⁺ DN TCRβ⁺ cells. Dotted lines represent CD1d^−/−MHCII^−/−, and solid lines represent Jα18^−/−MHCII^−/− mice. Shaded area shows the FMO for background fluorescence. Inlet bar diagrams within the respective histograms are summaries of 6 mice from three independent experiments, and are expressed as mean frequencies of positive cells ± SD. White bars represent CD1d^−/−MHCII^−/− mice, and black bars Jα18^−/−MHCII^−/− mice. Statistics were analysed using Mann‐Whitney U test (two‐tailed)