Skip to main content
. 2019 May 28;597(13):3441–3455. doi: 10.1113/JP277615

Figure 1. Cortical spreading potentiation during right hindpaw ischaemia with or without the spinal application of l‐NAME.

Figure 1

A, experimental method. B, cortical responses in the right somatosensory cortex elicited by brush vibration applied to the sole of the left hindpaw. In these mice, 5 μl saline was intrathecally applied to the spinal cord before the recordings. Original and pseudocolour images in ΔF/F 0 of the right somatosensory cortices are shown. The time after the onset of ischaemia in the right hindpaw is shown in each pseudocolour image. The white square in the left‐most pseudocolour image represents the ROI for the measurement of the response amplitude in ΔF/F 0. The pseudocolour scale shows the percentages of ΔF/F 0. C, cortical somatosensory responses elicited by hindpaw stimulation before and after the onset of ischaemia in the right hindpaw. In these mice, l‐NAME (5 μl, 100 mM) was applied to the spinal cord. Original and pseudocolour images in ΔF/F 0 are shown. D, relative amplitudes of the cortical responses normalized by those recorded before the onset of ischaemia. Mean and SEM are shown. Individual data are also shown by dots. The scale in the bar graphs represents the relative amplitude of the responses as a percentage. [Color figure can be viewed at http://wileyonlinelibrary.com]