Figure 7.

Estrogen-related receptor γ (ERRγ) directly activates the transcription of Mmp9 and Vegfa in mandibular condylar chondrocytes (MCCs), and U0126 and GSK5182 exert significant inhibitory effects on angiogenesis. (A) and (B) Quantitative Mmp9 and Vegfa chromatin immunoprecipitation (ChIP) assays were performed, and the data are presented as fold changes relative to each input (n = 3). (C) and (D) The ERRE sequences in the promoter regions of Mmp9 and Vegfa are indicated. (E) and (F) ChIP assays showing the binding of ERRγ to the Mmp9 and Vegfa promoters. Numbers on the right indicate the distance from the transcription start site. MCCs were treated with interleukin 6 (IL-6) (20 ng/ml) for 12 h, and soluble chromatin was immunoprecipitated with an ERRγ-specific antibody (n = 3). (G–I) Representative images and pooled data in the graph show the appearance of blood vessels in chick embryo chorioallantoic membranes (CAMs) treated with DMSO, IL-6 (20 ng/ml), IL-6+U0126 (50 μM), or IL-6+GSK5182 (50 μM) for 24 h (n = 3). All experiments were performed in triplicate, and the results are expressed as the mean ± SD. *P < 0.05; **P < 0.01. Scale bar: 50 μm.