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. 2019 Nov 6;10:1290. doi: 10.3389/fphar.2019.01290

Figure 8.

Figure 8

Sufficiency experimental designed to verify the inhibitory effects of Estrogen-related receptor γ (ERRγ) small interfering RNAs (siRNAs), U0126, and GSK5182 on temporomandibular joint osteoarthritis (TMJOA). (A) Western blots reveal certain therapeutic effects of U0126, GSK5182, and ERRγ siRNAs on TMJOA, and HIF-1α and ERRα do not reverse the effects of extracellular matrix degradation and vascularization on TMJOA (n = 3). (B) CCK-8 assay was used to examine the effect of GSK5182 on cell proliferation (n = 3). (C) Images of toluidine blue staining of mandibular condylar chondrocytes (MCCs) (P2) treated with interleukin 6 (IL-6) (20 ng/ml) for 12 h and stimulated with U0126 or GSK5182 (10 μM) for 12 h (n = 3). (D) MCCs transiently transfected with pcDNA vector or HIF-1α cDNA constructs were cultured in the absence or presence of IL-6. Western blots of MCC lysates showed a decrease in IL-6-induced matrix metalloproteinase 13 (MMP13) levels in cells overexpressing HIF-1α (n = 3). (E) Schematic showing the catabolic effects of the IL6-MAPK/extracellular signal–regulated kinase (ERK)–ERRγ axis on human chondrocytes. All experiments were performed in triplicate, and the results are expressed as the mean ± SD. *P < 0.05; **P < 0.01. NS not significant. Scale bar: 50 μm.