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. 2019 Aug 9;37(10):1293–1306. doi: 10.1002/stem.3057

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© 2019 The Authors. stem cells published by Wiley Periodicals, Inc. on behalf of AlphaMed Press 2019

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Human pluripotent stem cell‐derived neuronal differentiation systems exhibit cell stage‐ and cell state‐associated phenotypic heterogeneity. (A): Schematic outline of the neural induction and differentiation protocols used to derive proliferating neural stem cells (NSCs) and differentiated neurons (NEU) from pluripotent stem cell (PSC) sources. (B): Immunocytochemistry for the NSC marker SOX2 (red) costained with the neural differentiation marker TUJ1 (green) on human embryonic stem cell‐ as well as hiPSC‐derived proliferating NSC and differentiated NEU. Scale bars: 50 μm. (C): Western blot analysis for expression of key cell signaling pathway proteins (MAPK, AKT, JNK, hippo), neural differentiation (synapsin, MAP2, TUJ1, peripherin, ZEB1, GFAP), and proliferation (MYCN, ZO‐1) markers.