Figure 6.

Selective inhibition of DPAP1 or DPAP3 in live parasites. Intact mature schizonts were pretreated for 30 min with increasing concentrations of inhibitor followed by 1 h treatment with FY01. Samples were then run in an SDS/PAGE gel and the in‐gel fluorescence measured using a fluorescence scanner. (A) Representative gel images obtained for each of the inhibitors. Fluorescent bands corresponding to the different cysteine proteases labelled by the probe are indicated with arrowheads (DPAP3, red; FPs, green; DPAP1, blue). (B) The fluorescence intensity of each of the indicated bands was quantified by densitometry using ImageJ, and the fluorescence values normalized to the DMSO control. IC ₅₀ values are reported in Table 5. Note that control inhibitors containing D‐Trp are more than 1000‐fold less potent than those with L‐Trp. Three technical replicates were performed; dose responses for a representative replicate are shown.