Table 2.

DPAP3, DPAP1 and CatC inhibition constants for the vinyl sulfone library

P2	DPAP3			DPAP1			CatC
	k inact ·103 (s−1)	K i (nm)	k inact/K i·10‐3 (m −1·s−1)	k inact ·103 (s−1)	K i (nm)	k inact/K i·10‐3 (m −1·s−1)	k inact ·103 (s−1)	K i (nm)	k inact/K i·10−3 (m −1·s−1)
Gly	4.2 ± 0.3	3300 ± 750	1.30 ± 0.25	3.0 ± 0.2	3300 ± 750	0.9 ± 0.1	3.0 ± 0.2	70 ± 9	43 ± 4
Ala	2.4 ± 0.1	88 ± 13	27 ± 3	3.03 ± 0.09	90 ± 7	34 ± 2	3.00 ± 0.06	9.7 ± 0.4	310 ± 8
Acpc	3.2 ± 0.1	47 000 ± 3000	0.0677 ± 0.0001	6.2 ± 0.3	49 000 ± 4000	0.127 ± 0.006	1.03 ± 0.06	3200 ± 700	0.33 ± 0.06
hAla	2.2 ± 0.3	13.5 ± 6	166 ± 50	5.2 ± 0.7	19 ± 4	267 ± 26	2.9 ± 0.2	5.9 ± 0.9	500 ± 50
Aib	N.S.	N.S.	0.0363 ± 0.0006	8.9 ± 0.5	18 000 ± 2000	0.49 ± 0.03	1.20 ± 0.07	2100 ± 300	0.57 ± 0.06
Val	3.2 ± 0.2	5.7 ± 1.1	554 ± 84	3.6 ± 0.2	2.6 ± 0.4	1380 ± 140	2.9 ± 0.1	14 ± 2	200 ± 15
Ile	3.4 ± 0.4	23 ± 9	148 ± 45	3.2 ± 0.1	92 ± 8	34 ± 3	3.7 ± 0.8	1300 ± 400	2.9 ± 0.3
nLeu	3.7 ± 0.2	31 ± 3	120 ± 7.5	4.4 ± 0.3	14 ± 2	305 ± 24	2.40 ± 0.05	7.0 ± 0.5	340 ± 20
Cba	2.5 ± 0.1	140 ± 25	17 ± 2.5	5.4 ± 0.4	13 000 ± 2000	0.41 ± 0.03	1.29 ± 0.03	2400 ± 140	0.53 ± 0.02
Thr	2.0 ± 0.1	36 ± 8	55 ± 10	4.4 ± 0.2	112 ± 10	39 ± 2	2.38 ± 0.03	65 ± 3	36 ± 1
Asn	7.4 ± 0.8	2100 ± 1000	3.5 ± 1.4	9.9 ± 0.5	10 500 ± 750	0.95 ± 0.02	2.40 ± 0.07	260 ± 30	9.2 ± 0.7
Gln	2.9 ± 0.2	80 ± 20	35 ± 8	4.7 ± 0.2	50 ± 3	93 ± 3	2.3 ± 0.1	15 ± 3	160 ± 25
Asp	2.9 ± 0.2	1900 ± 400	1.5 ± 0.2	5.4 ± 0.2	2100 ± 200	2.6 ± 0.10	3.0 ± 0.1	2200 ± 200	1.4 ± 0.2
Glu	2.7 ± 0.1	51 ± 8	53 ± 6	5.0 ± 0.4	650 ± 100	7.6 ± 0.75	2.6 ± 0.1	140 ± 20	18 ± 2
His	3.7 ± 0.3	100 ± 25	35.5 ± 7.5	4.3 ± 0.3	150 ± 20	29 ± 3	2.1 ± 0.1	0.6 ± 0.1	3350 ± 500
Lys	N.S.	N.S.	0.020 ± 0.001	N.S.	N.S.	0.081 ± 0.004	1.49 ± 0.05	3600 ± 200	0.42 ± 0.02
Arg	N.S.	N.S.	0.065 ± 0.006	N.S.	N.S.	0.084 ± 0.002	4.0 ± 0.5	11 000 ± 2000	0.38 ± 0.02
Phg	N.S.	N.S.	0.0357 ± 0.0007	8.3 ± 0.5	7000 ± 650	1.19 ± 0.05	2.5 ± 0.2	86 ± 20	29 ± 5
2fa	3.8 ± 0.3	39 ± 10	97 ± 18	5.2 ± 0.4	11 ± 3	470 ± 90	3.7 ± 0.2	2.1 ± 0.2	1720 ± 90
2ta	3.4 ± 0.3	29 ± 7	118 ± 23	3.8 ± 0.3	82 ± 20	47 ± 7	3.3 ± 0.3	6.5 ± 1	510 ± 55
Cha	2.7 ± 0.1	90 ± 20	31.6 ± 5.5	N.S.	N.S.	0.020 ± 0.001	2.3 ± 0.1	300 ± 40	7.7 ± 0.8
Phe	2.6 ± 0.2	45 ± 13	60 ± 15	N.S.	N.S.	0.40 ± 0.01	2.18 ± 0.08	1.4 ± 0.2	1600 ± 200
Phe(Me)	5.4 ± 0.4	9200 ± 300	0.580 ± 0.025	N.S.	N.S.	0.314 ± 0.005	2.8 ± 0.2	1300 ± 200	2.2 ± 0.3
Phe(I)	3.8 ± 0.3	110 ± 30	36 ± 10	7.0 ± 0.3	47 000 ± 3500	0.151 ± 0.006	1.02 ± 0.06	185 ± 40	5.5 ± 0.9
Phe(NH2)	3.1 ± 0.2	39 ± 7	80 ± 12	5.0 ± 0.4	22 000 ± 2500	0.23 ± 0.01	1.01 ± 0.02	5.3 ± 0.3	191 ± 9
Tyr	2.18 ± 0.07	12.7 ± 1.5	172 ± 16	N.S.	N.S.	0.310 ± 0.005	2.10 ± 0.06	11.5 ± 0.9	182 ± 10
Tyr(NO2)	7.2 ± 0.9	250 ± 40	28.8 ± 0.8	N.S.	N.S.	0.023 ± 0.002	N.S.	N.S.	0.147 ± 0.008
hPhe	2.36 ± 0.08	310 ± 30	7.6 ± 0.7	N.S.	N.S.	0.029 ± 0.002	N.S.	N.S.	0.103 ± 0.005
Trp	2.5 ± 0.1	30 ± 6	84 ± 14	N.S.	N.S.	0.074 ± 0.002	1.98 ± 0.03	61 ± 4	32 ± 2
Igl	2.2 ± 0.1	29 ± 5	78 ± 12	N.S.	N.S.	0.313 ± 0.007	2.3 ± 0.1	240 ± 40	10 ± 1
2Nal	3.7 ± 0.3	7100 ± 1000	0.510 ± 0.04	5.3 ± 1.3	15 200 ± 6500	0.35 ± 0.07	2.6 ± 0.2	1800 ± 400	1.5 ± 0.2
Bip	N.S.	N.S.	0.0314 ± 0.0004	N.S.	N.S.	0.0178 ± 0.0004	2.53 ± 0.09	6200 ± 800	0.41 ± 0.04
Inp	5.2 ± 0.4	65 000 ± 800	0.079 ± 0.004	N.S.	N.S.	0.03 ± 0.001	1.05 ± 0.06	5600 ± 900	0.19 ± 0.02
Amc	5.7 ± 0.3	75 000 ± 7000	0.076 ± 0.003	5.8 ± 0.3	27 000 ± 2500	0.213 ± 0.009	0.97 ± 0.04	1000 ± 200	0.94 ± 0.17
3Abz	3.4 ± 0.4	3100 ± 600	1.1 ± 0.1	4.8 ± 0.3	3000 ± 500	1.6 ± 0.2	1.08 ± 0.07	55 ± 12	20 ± 4
Amb	N/A	3200 ± 1000	N/A	N.S.	N.S.	0.140 ± 0.007	1.6 ± 0.1	2100 ± 300	0.74 ± 0.06

N.S. No saturation, only k _inact/ K _i could be obtained. N/A, no time‐dependent inactivation was observed, data consistent with reversible inhibition. Standard error of the global fit for each parameter and inhibitor obtained by fitting k _obs values (1 or 2 technical replicates for DPAP3, single replicates for DPAP1 and CatC) to Eqs. 6 or 7 are shown.