Table 3.
Steady‐state Michaelis–Menten parameters for CatC
| Substrate | N | k cat (s−1) | K m (μm) | k cat/K m ·10‐3 (m −1·s−1) |
|---|---|---|---|---|
| Ala‐hPhe‐ACC | 3 | 2.13 ± 0.07 | 20 ± 2 | 105 ± 7 |
| Arg‐hPhe‐ACC | 3 | 0.26 ± 0.05 | 90 ± 25 | 2.8 ± 0.4 |
| Asp‐hPhe‐ACC | 3 | N.S. | N.S. | 1.2 ± 0.1 |
| Glu‐hPhe‐ACC | 3 | N.S. | N.S. | 3.8 ± 0.1 |
| Asn‐hPhe‐ACC | 3 | N.S. | N.S. | 6.0 ± 0.3 |
| Gln‐hPhe‐ACC | 3 | 1.52 ± 0.08 | 59 ± 5 | 26 ± 1 |
| Gly‐hPhe‐ACC | 3 | 1.6 ± 0.1 | 100 ± 10 | 16.1 ± 0.8 |
| His‐hPhe‐ACC | 3 | 1.23 ± 0.05 | 8 ± 1 | 150 ± 14 |
| Ile‐hPhe‐ACC | 3 | 0.21 ± 0.03 | 68 ± 15 | 3.1 ± 0.3 |
| Phe‐hPhe‐ACC | 3 | 0.68 ± 0.03 | 4.5 ± 0.5 | 150 ± 15 |
| Pro‐hPhe‐ACC | 3 | N.S. | N.S. | 1.9 ± 0.1 |
| Thr‐hPhe‐ACC | 3 | 0.68 ± 0.03 | 43 ± 3 | 15.8 ± 0.7 |
| Trp‐hPhe‐ACC | 3 | 0.30 ± 0.05 | 56 ± 20 | 534 ± 0.9 |
| Tyr‐hPhe‐ACC | 3 | 0.37 ± 0.02 | 28 ± 3 | 13.3 ± 0.8 |
| Val‐hPhe‐ACC | 3 | 1.45 ± 0.08 | 32 ± 4 | 46 ± 4 |
| hPhe‐hPhe‐ACC | 3 | N.A. | N.A. | N.A. |
| Cha‐hPhe‐ACC | 3 | 0.022 ± 0.003 | 4.5 ± 1.5 | 5 ± 1 |
| nLeu‐hPhe‐ACC | 3 | 1.92 ± 0.08 | 12 ± 1 | 160 ± 15 |
| Phg‐hPhe‐ACC | 3 | N.S. | N.S. | 0.58 ± 0.05 |
| Bip‐hPhe‐ACC | 3 | N.S. | N.S. | 0.8 ± 0.2 |
| Igl‐hPhe‐ACC | 3 | 0.11 ± 0.02 | 15 ± 4 | 7.4 ± 0.9 |
| Phe(Me)‐hPhe‐ACC | 3 | 0.084 ± 0.008 | 4.5 ± 1 | 19 ± 3 |
| 2Nal‐hPhe‐ACC | 3 | N.A. | N.A. | N.A. |
| hAla‐hPhe‐ACC | 3 | 2.05 ± 0.08 | 5.5 ± 0.8 | 372 ± 40 |
| nVal‐hPhe‐ACC | 3 | 2.06 ± 0.04 | 12.3 ± 0.6 | 167 ± 6 |
| hPro‐hPhe‐ACC | 3 | 0.8 ± 0.1 | 150 ± 30 | 5.4 ± 0.3 |
N is the number of replicates. N.A. No activity measured up to 100 μm substrate. Standard errors are shown for each parameter.