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. 2019 Jun 11;112(3):766–784. doi: 10.1111/mmi.14319

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© 2019 The Authors. Molecular Microbiology Published by John Wiley & Sons Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Left and right arm localisation when initiation of DNA replication is blocked via the temperature‐sensitive dna‐1 mutant. Distances between the +87° and −87° arm were examined in outgrown spores of dna‐1 (SU895) and dna‐1 Δsoj‐spo0J (SU897). Graphs show distance between the +87° and −87° foci in the three different nucleoid morphologies: (A) single‐lobed, (B) bilobed, and (C) spread. Asterisks indicate statistically significant (Kolmogorov‐Smirnov test) differences in focal distances between mutants at P < 0.05, as determined in from two replicate experiments. D. Representative images of +87° and −87° arm foci localisation including: (i) phase‐contrast and DAPI overlay, (ii) +87° and −87° foci overlay, and (iii) +87° and −87° foci overlaid with DAPI. Arrows highlight the positions of the +87° (cyan arrows) and −87° (yellow arrows) foci on each arm. Scale bar represents 2 μm. n > 100 for each strain.