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. 2019 Jun 11;112(3):766–784. doi: 10.1111/mmi.14319

Figure 5.

Figure 5

Z ring positioning and nucleoid morphology analysis when SMC is degraded. A. Nucleoid morphologies and B. midcell Z ring frequencies when SMC is degraded in outgrown temperature‐sensitive dna‐1 mutant spores via the addition of xylose (1% v/v) 15 min prior to shifting the cells to the non‐permissive temperature. C. Immunoblot analysis of SMC and a loading control (FtsZ) in wild‐type (SU5) and the smc‐ssrA degradation strains (dna‐1; SU851, Δnoc; SU875, Δsoj‐Δspo0J; SU877, Δsoj‐Δspo0J Δnoc; SU879) under conditions in which the adaptor protein is either not induced (−) or induced (+). n > 200 for each strain.