Figure 3.

Influence of the neural blocker TTX followed by DKT treatment on intraluminal pressure in the isolated rat proximal colons. A‐B, A typical pattern of intraluminal pressure in the proximal colons after the addition of vehicle (A) and the neural blocker TTX (0.3 μmol/L) (B) under DKT (200 μg/mL) treatment. C‐E, The change in intraluminal pressure in the proximal colons treated with vehicle or TTX is expressed by the ratio of after and before treatment. Compared with vehicle treatment, TTX significantly decreased PA (A) and AUC (C), and increased PF (B) of the intraluminal pressure in the proximal colons that were pretreated with DKT. Each point is represented as mean ± standard error (n = 4‐5). †, ‡, *: P < .05, .01, .001 for TTX vs. vehicle treatment, according to the Student t test or Aspin‐welch t test. AUC: area under the curve of pressure waves; PA: mean peak amplitude; PF, peak frequency; DKT, daikenchuto; TTX, tetrodotoxin