Figure 4.

Effect of Poly I:C and/or Pam3CSK4 on gene expression and protein levels of MCP‐1 in hPDLSCs. Primary hPDLSCs were stimulated with single TLR agonist (blue bars, concentrations are given in µg/ml) or their combinations (red bars) for 24 hours. Resulting gene expression levels of MCP‐1 were measured with qPCR and are represented as n‐fold expression in relationship to untreated cells (A). Protein concentration of MCP‐1 in the conditioned media (B) was analyzed with ELISA and is represented in B. All data are presented as mean ± SEM of 10 values obtained in independent experiments on hPDLSCs from 10 different healthy donors. ^*Significantly higher versus control (untreated cells), P < 0.05. ^†Significantly different between simultaneous stimulation and corresponding single stimulations, P < 0.05