Fig. 3.

Yoda-1 and shear stress both produce anti-inflammatory phenotypes in endothelium treated with TNF-α. (A–D) Experiments were performed in a similar manner to Fig. 1, but 100 U/mL TNF-α was introduced at the 20 h point for the remaining 4 h. ICAM-1 (A–B) or VCAM-1 (C–D) surface levels quantified (MFI values normalised against their appropriate unstimulated control). (A,C) One-way ANOVA with Tukey’s post-test. *P < 0.01, ##P < 0.001. (B,D) One-way ANOVA with Dunnett’s post test conducted, compared to DMSO (vehicle) control ##P < 0.001 ###P < 0.0001 or TNF-α alone ***P < 0.0001.