Fig. 5.
Yoda-1 treatment does not induce f-actin alignment and cell elongation. HUVEC were treated under static conditions for 24 h (1 μM Yoda-1 or DMSO), or under shear stress continuously (5 dyn/cm2) or a non-continuous low shear stress (0.5 dyn/cm2; 1 min flow cycle per 2 h). To visualise f-actin, cells were stained with phalloidin. (A) Representative confocal pictures for each condition. Arrow indicates the direction of media flow across the endothelium. Scale bar = 25 μm. (B) Directionality of f-actin fibres (relative to the direction of media flow when indicated by a black arrow) For each condition, three randomly selected fields of view were chosen for analysis. Data represent five independent repeats. (C–D) Elongation ratio (cell minor axis length)/ (cell major axis length). Non-paired students t-test conducted for the respective static and shear stress conditions ***P < 0.0001.