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Primers used for genotyping of bovine wild‐type cell lines, edited colonies and cloned animals
| Oligo | Sequence (5′‐3′) | Gene | Target exon | Amplicon (bp) |
|---|---|---|---|---|
| FW1 | GGATGCCTTTGATAGAGTTGG | GGTA1 | 9 | 440 |
| RV1 | GCTTTCATCATGCCATTGG | |||
| FW2 | AGCATCTTTCACAACTCAGG | GGTA1 | 4 | 739 |
| RV2 | TGAGACATTAGGAACATGGC | |||
| FW3 | TCAGGAGGAGACATCACCAACGG | CMAH | 2 | 225 |
| RV3 | TGCCCATCCTACTTGTCGAGGG |
