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. 2019 May 22;26(5):e12524. doi: 10.1111/xen.12524

Table 2.

Oligonucleotides synthetized for the assembly of desired CRISPR/Cas9 expression vectors used for the male line and sequence of the ssCMAH‐STOP oligo

Oligo Sequence (5′‐3′) Guide sequence—PAM (5′‐3′) Target gene (exon) Expression vector
btGGTA1cr1 FW CACCGGAGACCCTGGGCGAGTCGG GGAGACCCTGGGCGAGTCGG‐TGG GGTA1 (9) pX330‐btGGTA1cr1
btGGTA1cr1 RV AAACCCGACTCGCCCAGGGTCTCC
btGGTA1cr2 FW CACCGCTGGGCCACCGACTCGCCC GCTGGGCCACCGACTCGCCC‐AGG GGTA1 (9) pX330‐btGGTA1cr2
btGGTA1cr2 RV AAACGGGCGAGTCGGTGGCCCAGC
btCMAHcr1FW CACCGACTATGGGCAGGCAAGTGA GACTATGGGCAGGCAAGTGA‐GGG CMAH (2) pX330‐btCMAHcr1
btCMAHcr1 RV AAACTCACTTGCCTGCCCATAGTC
ssCMAH‐STOP oligo GTGACAGCTGCCATTCTTCTGAAATACCCAGGGAGAGGCAACGACAGACTTAAGGCAGGCAAGTGAGGGAGGCATTACTTTGCTGGGAAGGTGGGGTCAA // // //