Table 2.
Oligo | Sequence (5′‐3′) | Guide sequence—PAM (5′‐3′) | Target gene (exon) | Expression vector |
---|---|---|---|---|
btGGTA1cr1 FW | CACCGGAGACCCTGGGCGAGTCGG | GGAGACCCTGGGCGAGTCGG‐TGG | GGTA1 (9) | pX330‐btGGTA1cr1 |
btGGTA1cr1 RV | AAACCCGACTCGCCCAGGGTCTCC | |||
btGGTA1cr2 FW | CACCGCTGGGCCACCGACTCGCCC | GCTGGGCCACCGACTCGCCC‐AGG | GGTA1 (9) | pX330‐btGGTA1cr2 |
btGGTA1cr2 RV | AAACGGGCGAGTCGGTGGCCCAGC | |||
btCMAHcr1FW | CACCGACTATGGGCAGGCAAGTGA | GACTATGGGCAGGCAAGTGA‐GGG | CMAH (2) | pX330‐btCMAHcr1 |
btCMAHcr1 RV | AAACTCACTTGCCTGCCCATAGTC | |||
ssCMAH‐STOP oligo | GTGACAGCTGCCATTCTTCTGAAATACCCAGGGAGAGGCAACGACAGACTTAAGGCAGGCAAGTGAGGGAGGCATTACTTTGCTGGGAAGGTGGGGTCAA | // | // | // |