Figure 6.

ALS3 represses STOP1 accumulation in root nuclei.

Wild‐type (WT) and als3 mutant seedlings carrying the pSTOP1::GFP‐STOP1 construct were grown for 3 days on a phosphate‐ and Fe‐depleted (–Pi–Fe) plate (made with Seakem agar), transferred to –Pi–Fe or –Pi + 60 μm Fe plates for 2 h, and GFP fluorescence was visualized by confocal microscopy. To avoid saturated GFP fluorescence in the images, the microscope was set on the highest fluorescence (as detected in als3). Bars = 100 μm.