Table 1.
Specificity of carbohydrates targeting natural Abs in the group of mice exposed to B. longum, L. reuteri, B. thetaiotaomicron, a mixture of four strains of bacteria (B. longum, L. reuteri, B. thetaiotaomicron and E. coli) and mice put to normal food. The list of glycans with binding signals above 1500 relative fluorescence units (RFU) is presented.
| Glycan | Number of mice showing significant values of RFU | Glycan | Number of mice showing significant values of RFU |
|---|---|---|---|
| Group of mice exposed to B. longum NCC 2705 | Group of mice exposed to a mixture of four strains of bacteria (E. coli W3110, B. longum NCC 2705, B. thetaiotaomicron VPI-5482 (ATCC 29148), L. reuteri SD 2112 (ATCC 55730)) | ||
| GlcAβ1-6Galβ | 1 | ||
| Galα1-3(Fucα1-2)Galβ1-3GalNAcα | 1 | GalNAcβ | 3 |
| Galα1-3(Fucα1-2)Galβ1-3GalNAcβ | 1 | Rhaα | 1 |
| GalNAcα1-3(Fucα1-2)Galβ1-3GalNAcα | 1 | GalNAcβ | 2 |
| GalNAcα1-3(Fucα1-2)Galβ1-3GalNAcβ | 1 | (6-O-Bn-Galβ1)-3(6-O-Bn)GlcNAcβ | 2 |
| E. coli O58 | 1 | GlcAβ1-6Galβ | 1 |
| E. coli O127 | 2 | (GalNAcβ-PEG2)3-β-Asp-Asp | 3 |
| E. coli O148 | 1 | GalNAcα1-3(Fucα1-2)Galβ1-4GlcNAcβ | 1 |
| Sh. dysenteriae type 1 | 1 | GalNAcα1-4(Fucα1-2)Galβ1-4GlcNAcβ | 2 |
| Group of mice exposed to L. reuteri SD 2112 (ATCC 55730) | GalNAcα1-3(Fucα1-2)Galβ1-3GalNAcα | 3 | |
| Rhaα | 1 | E. coli O127 | 3 |
| 3,4-O-Su2-Galβ1-4GlcNAcβ | 3 | E. coli O148 | 1 |
| GlcNAcβ1-6(GlcNAcβ1-4)GalNAcα | 1 | P. mirabilis O13 | 1 |
| GalNAcα1-3(Fucα1-2)Galβ1-3GalNAcα | 1 | Sh. dysenteriae type 7 | 1 |
| (GlcNAcβ1)3-3,4,6-GalNAcα | 1 | Group of mice exposed to B. thetaiotaomicron VPI-5482 (ATCC 29148) | |
| GalNAcα1-3(Fucα1-2)Galβ1-3GalNAcβ | 1 | ||
| E. coli O127 | 3 | GalNAcβ1-4GlcNAcβ | 1 |
| E. coli O148 | 1 | GalNAcβ1-4(6-O-Su)GlcNAcβ | 1 |
| P. mirabilis O28 | 1 | 3-O-Su-GalNAcβ1-4GlcNAcβ | 1 |
| P. mirabilis O31 | 1 | 6-O-Su-GalNAcβ1-4GlcNAcβ | 1 |
| Sh. dysenteriae type 1 | 1 | 3,6-O-Su2-GalNAcβ1-4GlcNAcβ | 3 |
| Group of mice put to non-sterile food | 6-O-Su-GalNAcβ1-4(6-O-Su)GlcNAcβ | 2 | |
| 3,4-O-Su2-Galβ1-4GlcNAcβ | 1 | GlcNAcβ1-6(GlcNAcβ1-4)GalNAcα | 1 |
| 3-O-Su-GalNAcβ1-4GlcNAcβ | 1 | GlсNAcβ1-4(Fucα1-6)GlcNAcβ | 1 |
| 3,6-O-Su2-GalNAcβ1-4GlcNAcβ | 2 | (GlcNAcβ1-4)6 | 1 |
| E. coli O58 | 1 | E. coli O127 | 1 |
| E. coli O127 | 1 | P. mirabilis O31 | 3 |
| P. aerginosa O4ac | 1 | ||