Table 2.
Specificity of carbohydrates targeting natural Abs in the group of mice orally gavaged with a sample of non-sterile mice feces (left columns, ligands positive for at minimum two of four mice are shown; total amount of glycans to which Abs were detected is 54, see Supplementary Table 1) and non-sterile, conventional mice (right columns, only ligands positive for at minimum three of four mice are shown; total amount of glycans to which Abs were detected is 93, see Supplementary Table 1). The list of glycans with binding signals above 1500 relative fluorescence units (RFU) is presented.
| Group of mice orally gavaged with a sample
of non-sterile mice feces |
Group of non-sterile mice |
||
|---|---|---|---|
| Glycan/bacterial strain | Number of mice showing significant values of RFU | Glycan/bacterial strain | Number of mice showing significant values of RFU |
| GlcNAcβ1-4GlcNAcβ | 3 | 3-O-Su-GlcNAcβ | 4 |
| Galα1-3(Fucα1-2)Galβ1-3GalNAcα | 2 | 6-Bn-Galβ1-4GlcNAcβ | 4 |
| Galα1-3Galβ1-4(Fucα1-3)GlcNAcβ | 2 | (6-O-Bn-Galβ1)-3(6-O-Bn)GlcNAcβ | 3 |
| Fucα1-2Galβ1-3GalNAcα | 4 | ||
| GalNAcα1-3(Fucα1-2)Galβ1-3GalNAcβ | 3 | GlcNAcβ1-3Galβ1-4GlcNAcβ | 3 |
| GlcNAcβ1-3Galβ1-4GlcNAcβ | 3 | ||
| GalNAcα1-3(Fucα1-2)Galβ1-3GalNAcα | 2 | Galβ1-4Galβ1-4GlcNAcβ | 3 |
| Galα1-3(Fucα1-2)Galβ1-3GalNAcα | 3 | ||
| (GlcNAcβ1-4)6 | 3 | Galβ1-3GlcNAcα1-6Galβ1-4GlcNAcβ | 3 |
| S. enterica O13 | 2 | Galβ1-3GlcNAcβ1-6Galβ1-4GlcNAcβ | 3 |
| S. enterica O67 | 3 | Galβ1-4GlcNAcα1-6Galβ1-4GlcNAcβ | 4 |
| S. enterica O41 | 2 | Galβ1-4GlcNAcβ1-6Galβ1-4GlcNAcβ | 3 |
| E. coli O58 | 4 | GalNAcα1-3(Fucα1-2)Galβ1-3GalNAcα | 4 |
| E. coli O127 | 4 | GalNAcα1-3(Fucα1-2)Galβ1-3GalNAcβ | 4 |
| E. coli O148 | 3 | Fucα1-2Galβ1-3GlcNAcβ1-3Galβ1-4GlcNAcβ | 3 |
| E. coli O14 | 2 | S. enterica O13 | 4 |
| E. coli O19ab | 2 | E. coli O58 | 4 |
| P. aerginosa O9ad | 2 | E. coli O127 | 4 |
| Sh. dysenteriae type 1 | 3 | E. coli O14 | 3 |
| Sh. flexneri type 6 | 2 | Sh. dysenteriae type 1 | 3 |