Figure 2.
F. tularensis replication in J774 cell lines. J774, J774.vec and J774.IL4 cells (5 × 105 per well) were incubated with doxycycline (50 ng/ml) for 12 h and subsequently infected with 5 × 106 LVS ((a) to (c)) or Schu S4 ((d) and (e)). Bacterial uptake and replication were measured 3 h and 24 h post-inoculation ((a) and (d)). Additionally, the IL-4 concentration in culture media was measured ((b) and (e)) and the cellular arginase activity was determined (C). ǂP < 0.05 between the indicated J774.IL4 and respective J774 and J774.vec groups.
LVS: live vaccine strain.