Figure 5.

NO-mediated control of LVS replication. J774, J774.vec and J774.IL-4 cells (1 × 10⁶ per well) were induced with doxycycline (50 ng/ml, 12 h). BMMØs derived from C57BL/6 mice were infected with BCG (1 × 10⁷) in the presence or absence of NO inhibitor L-NMMA, and NO levels in culture media measured 24 h post-inoculation (a). In a similar study, BMMØs and doxycycline-induced J774 cell lines were infected with BCG, or mock treated with medium in the presence or absence of L-NMMA for 48 h, and infected with LVS. NO concentration ((b), upper panel) and LVS replication ((b), lower panel) were measured 24 h post-LVS inoculation. *P < 0.05 between indicated groups. ǂP < 0.05 between the indicated J774.IL4 and respective J774 and J774.vec groups.

BCG: Bacillus Calmette Guẻrin; BMMØ: bone marrow-derived macrophages; L-NMMA: N^G-monomethyl-L-arginine acetate salt; LVS: live vaccine strain.