Figure 7.

Carcinogenic activity of SNHG1 was mediated through regulation of the mTORC2 pathway in vitro. (A) LoVo cells were transfected with si‐SNHG1 for 48 hours. P‐AKT, AKT, p‐SGK1, SGK1, p70S6K1, p‐p70S6K1, LC3 I, and LC3 II were detected in LoVo cells after transfection for 48 hours. (B) LoVo cells were transfected with miR‐137 inhibitor or miR‐137 mimics for 48 hours, respectively. P‐AKT, AKT, p‐SGK1, SGK1, p70S6K1, p‐p70S6K1, LC3 I, and LC3 II were detected in LoVo cells after transfection for 48 hours. (C) LoVo cells were subjected to MDA staining. GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase [Color figure can be viewed at wileyonlinelibrary.com]