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Maximum likelihood phylogenetic tree of 22 comammox Nitrospira amoA sequences. The arrow indicates the position of the outgroup, which consisted of two Nitrosomonas sequences. The Rifle comammox Nitrospira sequences are shown in red. Bootstrap support values ≥75%, ≥97% and =100% are indicated by white, grey and black circles, respectively. The scale bar corresponds to 10% estimated sequence divergence.
